Searchable abstracts of presentations at key conferences on reproductive biology and medicine

ra0001p113 | (1) | WCRB2014

Combinations of 17β-estradiol and androgen support bovine oocyte growth in vitro by maintaining physical connections with granulosa cells

Makita Miho , Miyano Takashi

Introduction: Recent studies have revealed the contribution of steroid hormones to in vitro growth of oocytes in different species. However, exactly how these steroid hormones support oocyte growth is not well understood. This study was conducted to examine the effect of various steroid hormones on in vitro growth of bovine oocytes and to determine the contribution of steroid hormones to oocyte growth by assessing the connections between oocytes and granulosa...

ra0001p058 | (1) | WCRB2014

O-GlcNAcylation in pig embryos during preimplantation development

Shibutani Mihiro , Miyano Takashi , Miyake Masashi

Protein modification with O-linked β-N-acetylglucosamine (O-GlcNAcylation) is essential for eukaryotic cells. There are many reports concerned with O-GlcNAcylateion, but little is known about it in preimplantation development. The objective of this study was to examine the presence of O-GlcNAcylation and the role of O-GlcNAc cycling in pig preimplantation development using parthenogenetic diploids. In-vitro matured ooc...

ra0001p049 | (1) | WCRB2014

Nucleoli are formed in developing mouse embryos without nucleolus precursor bodies

Kyogoku Hirohisa , Fulka Josef , Kitajima Tomoya S , Wakayama Teruhiko , Miyano Takashi

Introduction: The large, compact oocyte nucleoli, sometimes referred to as nucleolus precursor bodies (NPBs), are essential for embryonic development in mammals. It has been convincingly documented that zygotes inherit the oocyte nucleolar material and form NPBs again in pronuclei. During early embryonic development, the compact zygote NPBs gradually transform into reticulated nucleoli of somatic cells type. Here we show that zygote NPBs are not required for embryonic and full...